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MSDS

EGFR (L747-P753)S

Anti-EGFR(L747-P753)S Mouse Monoclonal Antibody
5/5

¥4,800.00

Cat.#:  26287

   Size:   100 μl

In Stock

          Product Description          

EGFR (L747-P753)S

货号:   26287
基因符号:  EGFR/HER1/ERBB1
描述:   EGFR(L747-P753)S 小鼠单克隆抗体
背景:   EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).
免疫原:   A synthetic peptide from the internal region of EGFR which includes the mutation of (L747-P753) S, human origin.
经过测试的应用:   ELISA, WB, IF, IHC
推荐稀释度：   
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100
浓度:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性：   Monoclonal
亚型:   
纯化::   Purified from ascites
Preservative: No
成分:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Recognizes EGFR(L747-P753)S, but not wild type EGFR protein from vertebrates.
储存条件::   Store at -20°C. Avoid repeated freezing and thawing

Western blot:  

Western blot analysis of recombinant EGFR(L747-P753) S and wild type proteins.

Purified His-tagged EGFR(L747-P753)S protein (702-987) and wild type protein (702-987) were blotted with EGFR(L747-P753)S mouse antibody (货号26287).

Immunofluorescence:  

Immunofluorescence of cells expressing EGFR proteins with EGFR(L747-P753)S antibody.

HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR(L747-P753)S plasmid (right column), then fixed and stained with EGFR(L747-P753)S monoclonal antibody (货号26287).

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