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EGFR(G719A)

Anti-EGFR(G719A) Mouse Monoclonal Antibody
5/5

¥4,800.00

Cat.#:  26115

   Size:   100 μl

In Stock

          Product Description          

EGFR(G719A)

货号:  26115
基因符号:  EGFR/HER1/ERBB1
描述:   EGFR(G719A) 小鼠单克隆抗体
背景:   EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC gamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).
免疫原:   A synthetic peptide from the internal region of EGFR which includes the mutation of G719A, human origin.
Tested 经过测试的应用:   ELISA, WB, IF, IHC
推荐稀释度:   
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100
浓度:    1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   
纯化::   Purified from ascites
Preservative: No
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Recognizes EGFR(G719A), but not wild type EGFR of vertebrates.
储存条件::  Store at -20°C. Avoid repeated freezing and thawing.
Western blot:  
Western blot analysis of recombinant EGFR(G719A) and wild type proteins.

Purified His-tagged EGFR(G719A) protein (aa 702-987) and wild type protein (aa 702-987) were blotted with EGFR(G719A) mouse antibody (cat. # 26115).

Immunofluorescence:  

Immunofluorescence of cells expressing EGFR proteins with EGFR(G719A) antibody.

HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR(G719A) plasmid (right column), then fixed and stained with EGFR (G719A) monoclonal antibody (cat. # 26115).