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Ran-GTP

Anti-Ran-GTP Monoclonal Antibody
5/5

¥4,800.00

Cat.#:  26915

   Size:   30 μl

In Stock

          Product Description          
RanGTP 小鼠单克隆抗体
货号:   26915
规格:  30 µL
基因符号:  RAN
描述:   Ran-GTP 小鼠单克隆抗体
背景:   Ran (Ras-related nuclear protein) is a member of the Ras-superfamily GTPases. Ran is involved in the transport of proteins across the nuclear envelope, as well as in microtubule organization during mitosis.
免疫原:   Recombinant full length protein of active Ran.
经过测试的应用:   IP, IHC and IF (Not applicable for WB since SDS denatures Ran GTPase)
引用文献的应用:  IP, IHC and IF – Click for Details
推荐稀释度:    IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250
浓度:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯化::   Purified from ascites
Preservative: No
成分:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Ran-GTP monoclonal antibody recognizes active Ran of vertebrates.
储存条件::  Store at -20°C. Avoid freeze/thaw cycles
Immunoprecipitation/Western blot:   Ran Activation Assay. Purified Ran proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the Ran-GTP monoclonal antibody (Cat. No. 26915). Immunoblot was with an Ran polyclonal antibody.
          Publications          
01. CFDP1 regulates the stability of pericentric heterochromatin thereby affecting RAN GTPase activity and mitotic spindle formation
PLoS Biol. 2024  PMID: 38630655
02. Mitosis-specific acetylation tunes Ran effector binding for chromosome segregation
J Mol Cell Biol. 2018  PMID: 29040603
03. OSBP-related protein 4L promotes phospholipase Cß3 translocation from the nucleus to the plasma membrane in Jurkat T-cells
J Biol Chem. 2018  PMID: 30237164
04. Initiation of DNA replication requires actin dynamics and formin activity
EMBO J. 2017  PMID: 28982779
05. SGK1 affects RAN/RANBP1/RANGAP1 via SP1 to play a critical role in pre-miRNA nuclear export: a new route of epigenomic regulation
Sci Rep. 2017  PMID: 28358001
06. Drosophila TIM Binds Importin a1, and Acts as an Adapter to Transport PER to the Nucleus
PLoS Genet., 2015  PMID: 25674790
07. Immunofluorescence methods in studies of the GTPase RAN and its effectors in interphase and in mitotic cells
Methods Mol Biol. 2014  PMID: 24470030
08. Activation of Ran GTPase by a Legionella Effector Promotes Microtubule Polymerization, Pathogen Vacuole Motility and Infection
PLoS Pathog., 2013  PMID: 24068924
09. IQGAP1 Protein Regulates Nuclear Localization of β-Catenin via Importin-β5 Protein in Wnt Signaling
J Biol Chem. 2013  PMID: 24196961
10. Calcium-calmodulin kinase I cooperatively regulates nucleocytoplasmic shuttling of CCTa by accessing a nuclear export signal
Mol Biol Cell. 2012  PMID: 22621903
11. NMDAR signaling facilitates the IPO5-mediated nuclear import of CPEB3
Nucleic Acids Res. 2012  PMID: 22730302