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CTCF(R339W)

Anti-CTCF(R339W) Mouse Monoclonal Antibody
5/5

¥4,800.00

Cat.#:  26326

   Size:   100 μl

In Stock

          Product Description          

CTCF(R339W) 

货号:  26326
基因符号:  CTCF; MRD21
描述:   CTCF(R339W) 小鼠单克隆抗体
背景:   Transcriptional repressor CTCF also known as 11-zinc finger protein or CCCTC-binding factor is a transcription factor that in humans is encoded by the CTCF gene. CTCF is involved in many cellular processes, including transcriptional regulation, insulator activity, recombination and regulation of chromatin architecture.
免疫原:   A synthetic peptide from the internal region of CTCF which includes the mutation of R339W, human origin.
经过测试的应用:   ELISA, WB, IHC
推荐稀释度：   
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100
浓度:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性：   Monoclonal
亚型:   IgG
纯化::   Purified from ascites
Preservative: No
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Recognizes R339W mutant, but not wild type CTCF of vertebrates.
储存条件::   Store at -20°C. Avoid repeated freezing and thawing

Western blot:  

Western blot analysis of recombinant CTCF (R339W) and wild type proteins.

Purified His-tagged CTCF (R339W) protein (lane 1) and corresponding wild type protein (lane 2) were blotted with CTCF(R339W) monoclonal antibody (货号26326).

Immunofluorescence:  

Immunofluorescence of cells expressing CTCF proteins with CTCF(R339W) antibody.

HEK293T cells were transfected with pCDNA3-GFP-CTCF (WT) plasmid (left column) or pCDNA3-GFP-CTCF (R339W) plasmid (right column), then fixed and stained with CTCF(R339W) monoclonal antibody (货号26326).

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