| 目录: 28446 |
| 产品名称: CD40(DM102) Rabbit Monoclonal Antibody |
| 基因符号: CD40; Bp50; CDW40; MGC9013; TNFRSF5; p50 |
| 描述: CD40 antibody(DM102) Rabbit Monoclonal Antibody |
| 背景: This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching; memory B cell development; and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand; which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation; and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |
| 经过测试的应用: ELISA; Flow Cyt |
| 推荐稀释度： ELISA 1:5000-10000; Flow Cyt 1:100 |
| 种属反应性: Rabbit |
| 亚型: Rabbit IgG |
| 纯化: Purified from cell culture supernatant by affinity chromatography |
| 种属反应性: Human CD40 |
| 成分: Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). |
Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD40 protein, mFc-His tagged protein 11143 can bind Rabbit CD40 monoclonal antibody ( clone: DM102) in a linear range of 0.128-16 ng/ml. | 
Figure 2. Flow cytometry analysis with CD40 ( DM102) on Expi293 cells transfected with human CD40 ( Blue histogram) or Expi293 transfected with irrelevant protein ( Red histogram). |
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