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ALK pAb

Anti-ALK Rabbit Polyclonal Antibody
5/5

¥2,600.00

Cat.#:  21280

   Size:   100 μl

In Stock

          Product Description          

ALK Polyclonal Antibody

货号:  21280
基因符号:   ALK, CD246, NBLST3
描述:   ALK Rabbit Polyclonal Antibody
背景:   Anaplastic lymphoma kinase (ALK) also known as ALK tyrosine kinase receptor or CD246 (cluster of differentiation 246) is a neuronal orphan receptor tyrosine kinase. ALK is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Abnormal ALK, by fusion with other genes, gaining additional gene copies or mutations, can be oncogenic.
免疫原:   Purified ALK protein, human origin.
经过测试的应用:   ELISA, WB, IHC
推荐稀释度：   
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:200
浓度:   0.2 mg/ml
种属反应性:   Rabbit
形式:   Liquid
克隆性：   Polyclonal
亚型:   IgG
纯化::   Purified from serum
Preservative: No
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Recognizes ALK of vertebrates.
储存条件::   Store at -20°C. Avoid repeated freezing and thawing

Western blot:  

Western blot analysis of recombinant ALK protein. Purified His-tagged ALK protein was blotted with ALK rabbit polyclonal antibody (货号21280).
Immunohistochemistry:  

Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with ALK polyclonal antibody (货号21280).

Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat Rabbit IgG (1:50 dilution) was used as secondary antibody.

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