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2B4 (DM70) Rabbit mAb

Anti-2B4 (DM70) Rabbit Monoclonal Antibody
5/5

¥3,900.00

Cat.#:  28381

   Size:   100 μg

In Stock

          Product Description          
目录:   28381
产品名称:   2B4(DM70) Rabbit Monoclonal Antibody
基因符号:   CD244;2B4;SLAMF4;NKR2B4;NAIL;h2B4
描述:    2B4 antibody(DM70) Rabbit Monoclonal Antibody
背景:    This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
经过测试的应用:    ELISA; Flow Cyt
推荐稀释度:    ELISA 1:5000-10000; Flow Cyt 1:100
种属反应性:    Rabbit
亚型:    Rabbit IgG
纯化:    Purified from cell culture supernatant by affinity chromatography
种属反应性:    Human 2B4
成分:    Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.
储存和运输:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human 2B4 protein, mFc-His tagged protein  11138][getskuurl sku=)] can bind Rabbit 2B4 monoclonal antibody ( clone: DM70) in a linear range of 1-100 ng/ml.

Figure 2. Expi 293 cell line transfected with irrelevant protein  (A) and human 2B4  (B) were surface stained with Rabbit 2B4 monoclonal antibody 1µg/ml ( clone: DM70) followed by Alexa 488-conjugated rabbit IgG secondary antibody.

Figure 3. Flow cytometry data of serially titrated Rabbit 2B4 monoclonal antibody ( clone: DM70) on THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Figure 4. Affinity ranking of different Rabbit 2B4 mAb clones by titration of different concentration onto THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
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