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SMAD4(R361S)

Anti-SMAD4(R361S) Mouse Monoclonal Antibody
5/5

¥4,800.00

Cat.#:  26423

   Size:   100 μl

In Stock

          Product Description          
SMAD4(R361S)
货号:  26423
基因符号:  SMAD4, DPC4, JIP, MADH4, MYHRS
描述:   SMAD4(R361S) 小鼠单克隆抗体
背景:   SMAD4 family member 4, also known as SMAD4, is a protein that in human is encoded by the SMAD4 gene. It is often found mutated in many cancers. It acts as a tumor suppressor that functions in the regulation of the TGF-? signal transduction pathway, which negatively regulates growth of epithelial cells and the extracellular matrix (ECM). SMAD4 alterations have been found in multiploid colorectal cancer and pancreatic carcinoma. It is found inactivated in at least 50% of pancreatic cancers. It is also found mutated in the autosomal dominantdisease juvenile polyposis syndrome (JPS).
免疫原:   A synthetic peptide from the internal region of SMAD4 which includes the mutation of R361S, human origin.
经过测试的应用:   ELISA, WB, IHC
推荐稀释度:    ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IHC: 1:50-1:100
浓度:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯化::   Purified from ascites
Preservative:  No
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Recognizes R361S mutant, but not wild type SMAD4 of vertebrates
储存条件::   Store at -20°C. Avoid repeated freezing and thawing
Western blot:   Western blot analysis of recombinant SMAD4(R361S) and wild type proteins. Purified His-tagged SMAD4(R361S) protein (lane1) and corresponding wild type protein (lane2) were blotted with SMAD4(R361S) monoclonal antibody (货号26423). Immunofluorescence:   Immunofluorescence of cells expressing SMAD4 proteins with SMAD4(R361S) antibody. HEK293T cells were transfected with pCDNA3-GFP-SMAD4(R361S) plasmid pCDNA3-GFP-SMAD4 (WT) plasmid, pCDNA3-GFP-SMAD4 (R361H) plasmid or pCDNA3-GFP-SMAD4 (R361C) plasmid, then fixed and stained with SMAD4(R361S) monoclonal antibody (货号26423).
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