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RheB(Y35N)

Anti-RheB(Y35N) Mouse Monoclonal Antibody
5/5

¥4,800.00

Cat.#:  26338

   Size:   100 μl

In Stock

          Product Description          
RheB(Y35N)
货号:  26338
规格:  100 µL
基因符号:  RHEB
描述:   RheB(Y35N) 小鼠单克隆抗体
背景:   RheB (Ras Homolog Enriched in Brain) is an evolutionarily conserved member of the Ras-family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, RheB is widely expressed in other tissues and may be induced by growth factor stimulation. RheB has an important role in regulating the insulin/TOR signaling pathway. The mammalian target of rapamycin, mTOR, is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with translation and cell growth. The tuberin/hamartin (TSC2/TSC1) complex inhibits mTOR activity indirectly by inhibiting RheB via tuberin’s GAP activity.
免疫原:   A synthetic peptide from the internal region of RHEB which includes the mutation of Y35N, human origin.
经过测试的应用:   ELISA, WB, IHC
推荐稀释度:    ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IHC: 1:50-1:100
浓度:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯化::   Purified from ascites
Preservative:  No
成分:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Recognizes Y35N mutant, but not wild type RHEB of vertebrates.
储存条件::   Store at -20°C. Avoid repeated freezing and thawing
Western blot:   Western blot analysis of recombinant RHEB(Y35N) and wild type proteins. Purified His-tagged RHEB(Y35N) protein (lane2) and corresponding wild type protein (lane1) were blotted with RheB(Y35N) monoclonal antibody (货号26338).
Immunofluorescence:   Immunofluorescence of cells expressing RHEB proteins with RHEB(Y35N) antibody. HEK293T cells were transfected with pCDNA3-GFP-RHEB (WT) plasmid (left column) or pCDNA3-GFP-RHEB(Y35N) plasmid (right column), then fixed and stained with RheB(Y35N) monoclonal antibody (货号26338).
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