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RAS(K117N)

Anti-RAS(K117N) Mouse Monoclonal Antibody
5/5

¥4,800.00

Cat.#:  26468

   Size:   100 μl

In Stock

          Product Description          
RAS(K117N) 
货号:  26468
基因符号:  H-Ras; K-Ras; N-Ras
描述:   RAS(K117N) 小鼠单克隆抗体
背景:   The K117N mutation of KRas results in an amino acid substitution at position 146, from an alanine to a threonine. KRAS encodes a protein that is a member of the small GTPase superfamily. Ras K117N mutation results in decreased GTPase activity and constitutive signaling. It can be found in many tumors, such as lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.
免疫原:   A synthetic peptide from the internal region of Ras which includes the mutation of K117N, human origin.
经过测试的应用:   ELISA, WB, IHC
推荐稀释度:    ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100
浓度:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯化::   Purified from ascites
Preservative: No
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Recognizes K117N mutant, but not wild type RAS of vertebrates.
储存条件::   Store at -20°C. Avoid repeated freezing and thawing
Western blot:   Western blot analysis of recombinant RAS(K117N) and wild type proteins. Purified His-tagged RAS(K117N) protein (lane 1) and corresponding wild type protein (lane 2) were blotted with RAS(K117N) monoclonal antibody (货号26468). Immunofluorescence:   Immunofluorescence of cells expressing KRas proteins with KRAS(K117N) antibody. HEK293T cells were transfected with pCDNA3-GFP-KRas (WT) plasmid (left column) or pCDNA3-GFP-KRAS(K117N) plasmid (right column), then fixed and stained with KRAS(K117N) monoclonal antibody (货号26468).