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RAS(G13R)

Anti-RAS(G13R) Mouse Monoclonal Antibody
5/5

¥4,800.00

Cat.#:  26474

   Size:   100 μl

In Stock

          Product Description          
RAS(G13R)
货号:  26474
基因符号:  H-Ras; K-Ras; N-Ras
描述:   RAS(G13R) 小鼠单克隆抗体
背景:   Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation, and cell survival. Ras G13R mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions.
免疫原:   A synthetic peptide from the internal region of Ras which includes the mutation of G13R, human origin.
经过测试的应用:   ELISA, WB, IHC
推荐稀释度:    ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100
浓度:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯化::   Purified from ascites
Preservative: No
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Recognizes G13R mutant, but not wild type RAS of vertebrates.
储存条件::   Store at -20°C. Avoid repeated freezing and thawing
Western blot:   Western blot analysis of recombinant HRAS(G13R) and wild type proteins. Purified His-tagged RAS(G13R) protein (lane 1) and corresponding wild type protein (lane 2) were blotted with RAS(G13R) monoclonal antibody (货号26474). Immunofluorescence:   Immunofluorescence of cells expressing Ras proteins with RAS(G13R) antibody. HEK293T cells were transfected with pCDNA3-GFP-RAS(G13R) plasmid,pCDNA3-GFP-Ras (WT) plasmid, pCDNA3-GFP-Ras (G13S) plasmid, pCDNA3-GFP-Ras (G13C) plasmid, pCDNA3-GFP-Ras (G13V) plasmid, pCDNA3-GFP-Ras (G13A) plasmid, or pCDNA3-GFP-Ras (G13D) plasmid, then fixed and stained with RAS(G13R) monoclonal antibody (货号26474).