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RAS(A146V)

Anti-RAS(A146V) Mouse Monoclonal Antibody
5/5

¥4,800.00

Cat.#:  26479

   Size:   100 μl

In Stock

          Product Description          
RAS(A146V)
货号:  26479
基因符号:  H-Ras; K-Ras; N-Ras
描述:   RAS(A146V) 小鼠单克隆抗体
背景:   The A146V mutation of KRas results in an amino acid substitution at position 146, from an alanine to a threonine. KRAS encodes a protein that is a member of the small GTPase superfamily. Ras A146V mutation results in decreased GTPase activity and constitutive signaling. It can be found in many tumors, such as lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.
免疫原:   A synthetic peptide from the internal region of Ras which includes the mutation of A146V, human origin.
经过测试的应用:   ELISA, WB, IHC
推荐稀释度:    ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100
浓度:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯化::   Purified from ascites
Preservative: No
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Recognizes A146V mutant, but not wild type RAS of vertebrates.
储存条件::   Store at -20°C. Avoid repeated freezing and thawing
Western blot:   Western blot analysis of recombinant RAS(A146V) and wild type proteins. Purified His-tagged RAS(A146V) protein (lane 1) and corresponding wild type protein (lane 2) were blotted with RAS(A146V) monoclonal antibody (货号26479). Immunofluorescence:   Immunofluorescence of cells expressing KRas proteins with KRAS(A146V) antibody. HEK293T cells were transfected with pCDNA3-GFP-KRAS(A146V) plasmid, pCDNA3-GFP-KRas (WT) plasmid, pCDNA3-GFP-KRas (A146P) plasmid or pCDNA3-GFP-KRas (A146T) plasmid, then fixed and stained with KRAS(A146V) monoclonal antibody (货号26479).
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