| 目录: 11012 |
| 产品名称: Human MDR-1 Full Length Protein |
| 规格: 10 µg, 50 µg and 100 µg |
| 基因符号: ABCB1; CD243; CLCS; GP170; MDR1; p-170; P-GP; PGY1 |
| Target: MDR-1 |
| UNIPROT ID: P08183 |
| 描述: Human MDR-1 full length protein-synthetic nanodisc |
| 背景: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. Mutations in this gene are associated with colchicine resistance and Inflammatory bowel disease 13. Alternative splicing and the use of alternative promoters results in multiple transcript variants. |
| Species/Host: HEK293 |
| Molecular Weight: The human full length MDR-1 protein has a MW of 141.5 kDa |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure1. Elisa plates were pre-coated with Flag Tag MDR-1-Nanodisc (0.2 µg/per well). Serial diluted Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for Flag monoclonal antibody binding with MDR-1-Nanodisc is 6.883ng/ml. | 
Figure2. Human MDR-1-Nanodisc, Flag Tag on SDS-PAGE |
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