| 目录: 12229 |
| 产品名称: Human CCR4 Full Length Protein |
| 规格: 10 µg, 50 µg and 100 µg |
| 基因符号: CC-CKR-4; CD194; ChemR13; CKR4; CMKBR4 |
| Target: CCR4 |
| UNIPROT ID: P51679 |
| 描述: Human CCR4 full length protein membrane nanoparticles (MNPs) |
| 背景: The protein belongs to the G-protein-coupled receptor family . It is a receptor for the CC chemokine – MIP-1, RANTES, TARC and MCP-1. Chemokines are a group of small polypeptide, structurally related molecules that regulate cell trafficking of various types of leukocytes. The chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. |
| Species/Host: HEK293 |
| Molecular Weight: The human full length CCR4 Protein has a MW of 41.4 kDa |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure1. Elisa plates were pre-coated with 0.5 µg/per well purified human CCR4 full length membrane nanoparticles. Serial diluted CCR4 monoclonal antibody (28089) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for CCR4 monoclonal antibody binding with CCR4 full length membrane nanoparticles is 308.3ng/ml. |    
Figure2. FACS analysis of CCR4 MNPs A. Negative Control 1: CCR4 full length membrane nanoparticles samples were stained only with Goat human lgG 488 secondary antibody. B. Negative Control 2: Control membrane nanoparticles samples were stained with CCR4 antibody (28089) at 2µg/ml, followed by Goat human IgG 488 secondary antibody. C. Negative Control 3: CCR4 full length membrane nanoparticles samples were stained with CCR8 antibody (an irrelevant antibody) at 2µg/ml, followed by Goat human IgG 488 secondary antibody. D. CCR4 full length membrane nanoparticles samples were stained with CCR4 antibody (28089) at 2µg/ml, followed by Goat human IgG 488 secondary antibody. |
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