武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处 

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13-GTP

Anti-Gα13-GTP Mouse Monoclonal Antibody
5/5

¥4,800.00

Cat.#:  26902

   Size:   30 μl

In Stock

          Product Description          
13GTP 小鼠单克隆抗体
货号: 26902
规格:  30 µL
基因符号:  Gna13
描述:   13-GTP 小鼠单克隆抗体
背景:   Heterotrimeric G proteins are essential cellular signal transducers. Gα13 is one of the G proteins that could mediate cell migration and angiogenesis. Other biochemical and physiological functions of Gα13 are being explored.
免疫原:   Recombinant full length active Gα13 protein
经过测试的应用:   IP, IHC and IF (Not applicable for WB since SDS denatures Gα13 GTPase)
引用文献的应用:  IF, IHC and IP – Click for Details
推荐稀释度:    IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250
浓度:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG2b
纯化::   Purified from ascites
Preservative:  No
成分:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  active Gα13 monoclonal antibody recognizes active Gα13 of vertebrates.
储存条件::  Store at -20°C. Avoid repeated freezing and thawing
Immunoprecipitation/Western blot:   IP/WB analysis of active Gα13 proteins. Purified Gα13 proteins were treated with GDP (lane 1) or GTPGTPγS (lane 2). After immunoprecipitation with active Gα13-GTP mouse monoclonal antibody (货号 26902), these proteins were separated by SDS/PAGE and western blotted with Gα13 mouse monoclonal antibody (货号 26004).
Immunohistochemistry:   Staining of MEF cells (treated with PDGF) with Gα13-GTP monoclonal antibody showing the localization of Gα13-GTP in the leading edge of migrating cells.