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ATM pAb

Anti-ATM Rabbit Polyclonal Antibody
5/5

¥2,600.00

Cat.#:  21276

   Size:   100 μl

In Stock

          Product Description          

ATM Polyclonal Antibody

货号:   21276
基因符号:  ATM
描述:   ATM Rabbit Polyclonal Antibody
背景:   Ataxia telangiectasia mutated (ATM) is a serine/threonine protein kinase that is recruited and activated by DNA double-strand breaks. It phosphorylates several key proteins that initiate activation of the DNA damage checkpoint, leading to cell cycle arrest, DNA repair or apoptosis. Activity of ATM protein is under tight control, and mutation of ATM can cause disease such as Ataxia telangiectasia (AT) and cancers.
免疫原:   Purified ATM protein, human origin.
经过测试的应用:   ELISA, WB, IHC
推荐稀释度：   
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:200
浓度:    0.2 mg/ml
种属反应性:   Rabbit
形式:   Liquid
克隆性：   Polyclonal
亚型:   
纯化::   Purified from serum
Preservative: No
成分:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Recognizes ATM of vertebrates.
储存条件::   Store at -20°C. Avoid repeated freezing and thawing

Western blot:  

Western blot analysis of recombinant ATM protein. Purified His-tagged ATM protein was blotted with ATM rabbit polyclonal antibody (货号21276).

Immunohistochemistry:  

Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with ATM polyclonal antibody (货号21276). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat mouse IgG (1:50 dilution) was used as secondary antibody.


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