Email: info@neweastbio.com   |  Call: 6109452007

NELFA Rabbit pAb

Anti-NELFA Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218285

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218285
Product Name:   Anti-NELFA Rabbit Polyclonal Antibody
Synonyms:   WHSC2; NELF-A; P/OKcl.15
UNIPROT ID:   Q9H3P2 (Gene Accession – BC002764 )
Background:    This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation.
Immunogen:    Fusion protein of human NELFA
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 218285(NELFA Antibody) at a dilution of 1/100(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the fusion protein and then with 218285(Anti-NELFA Antibody) at dilution 1/100.

The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using 218285(Anti-NELFA Antibody) at a dilution of 1/100.

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with fusion protein and then with D224094(Anti-NELFA Antibody) at dilution 1/100.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: A549 cell, Mouse testis tissue, Jurkat cell, RAW264.7 cell lysates;
Primary antibody: 218285(NELFA Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 15 seconds
          Publications