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EIF4G1 Rabbit pAb

Anti-EIF4G1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220518

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220518
Product Name:   Anti-EIF4G1 Rabbit Polyclonal Antibody
Synonyms:   P220; EIF4F; EIF4G; EIF4GI; PARK18; EIF-4G1
UNIPROT ID:   Q04637 (Gene Accession – NP_004944 )
Background:    The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5′-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.
Immunogen:    Synthetic peptide of human EIF4G1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 220518(EIF4G1 Antibody) at a dilution of 1/30(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the synthetic peptide and then with 220518(Anti-EIF4G1 Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 220518(Anti-EIF4G1 Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with synthetic peptide and then with D261641(Anti-EIF4G1 Antibody) at dilution 1/30.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Lovo cells, 293T cells;
Primary antibody: 220518(EIF4G1 Antibody) at dilution 1/450;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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