Cat.#: S220376 |
Product Name: Anti-COX5A Rabbit Polyclonal Antibody |
Synonyms: VA; COX; COX-VA |
UNIPROT ID: P20674 (Gene Accession – NP_004246 ) |
Background: Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. |
Immunogen: Synthetic peptide of human COX5A |
Applications: ELISA, WB, IHC |
Recommended Dilutions: IHC: 50-200;WB: 500-2000;ELISA: 5000-10000 |
Host Species: Rabbit |
Clonality: Rabbit Polyclonal |
Isotype: Immunogen-specific rabbit IgG |
Purification: Antigen affinity purification |
Species Reactivity: Human, Mouse, Rat |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
Research Areas: Metabolism |
Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 220376(COX5A Antibody) at a dilution of 1/40(cytoplasm). | In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the synthetic peptide and then with 220376(Anti-COX5A Antibody) at dilution 1/40. | The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 220376(Anti-COX5A Antibody) at a dilution of 1/40. | In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with synthetic peptide and then with D261450(Anti-COX5A Antibody) at dilution 1/40. | Gel: 12%SDS-PAGE, Lysate: 40 μg; Lane: 293T cell; Primary antibody: 220376(COX5A Antibody) at dilution 1/400; Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution; Exposure time: 7 seconds | |
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