Cat.#: S220039 |
Product Name: Anti-AGPAT2 Rabbit Polyclonal Antibody |
Synonyms: BSCL; BSCL1; LPAAB; 1-AGPAT2; LPAAT-beta |
UNIPROT ID: O15120 (Gene Accession – NP_001012745 ) |
Background: This gene encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. The protein is located within the endoplasmic reticulum membrane and converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis. Mutations in this gene have been associated with congenital generalized lipodystrophy (CGL), or Berardinelli-Seip syndrome, a disease characterized by a near absence of adipose tissue and severe insulin resistance. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
Immunogen: Synthetic peptide of human AGPAT2 |
Applications: ELISA, WB, IHC |
Recommended Dilutions: IHC: 50-200;WB: 500-2000;ELISA: 5000-10000 |
Host Species: Rabbit |
Clonality: Rabbit Polyclonal |
Isotype: Immunogen-specific rabbit IgG |
Purification: Antigen affinity purification |
Species Reactivity: Human |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
Research Areas: Cancer, Metabolism, Cardiovascular |
Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 220039(AGPAT2 Antibody) at a dilution of 1/50(Cytoplasm and Cell membrane). | In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the synthetic peptide and then with 220039(Anti-AGPAT2 Antibody) at dilution 1/50. | Gel: 12%SDS-PAGE, Lysate: 40 μg; Lane 1-4: K562, A549, 231, PC3 cell lysates; Primary antibody: 220039(AGPAT2 Antibody) at dilution 1/500; Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution; Exposure time: 1 minute | |
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