Cat.#: S220323 |
Product Name: Anti-AURKC Rabbit Polyclonal Antibody |
Synonyms: AIE2; AIK3; ARK3; AurC; SPGF5; STK13; aurora-C |
UNIPROT ID: Q9UQB9 (Gene Accession – NP_001015878 ) |
Background: This gene encodes a member of the Aurora subfamily of serine/threonine protein kinases. The encoded protein is a chromosomal passenger protein that forms complexes with Aurora-B and inner centromere proteins and may play a role in organizing microtubules in relation to centrosome/spindle function during mitosis. This gene is overexpressed in several cancer cell lines, suggesting an involvement in oncogenic signal transduction. Alternative splicing results in multiple transcript variants. |
Immunogen: Synthetic peptide of human AURKC |
Applications: ELISA, WB, IHC |
Recommended Dilutions: IHC: 15-50;WB: 200-1000;ELISA: 1000-2000 |
Host Species: Rabbit |
Clonality: Rabbit Polyclonal |
Isotype: Immunogen-specific rabbit IgG |
Purification: Antigen affinity purification |
Species Reactivity: Human, Mouse |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
Research Areas: Cancer |
Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human brain tissue using 220323(AURKC Antibody) at a dilution of 1/15(Cytoplasm). | In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with the synthetic peptide and then with 220323(Anti-AURKC Antibody) at dilution 1/15. | Gel: 10%SDS-PAGE, Lysate: 40 μg; Lane 1-5: Mouse testis tissue, hela cells, human liver cancer tissue, A375 cells, 293T cells; Primary antibody: 220323(AURKC Antibody) at dilution 1/150; Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution; Exposure time: 1 minute | |
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