Cat.#: S219636 |
Product Name: Anti-APAF1 Rabbit Polyclonal Antibody |
Synonyms: CED4, APAF-1 |
UNIPROT ID: O14727 (Gene Accession – NP_863659 ) |
Background: This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. |
Immunogen: Synthetic peptide of human APAF1 |
Applications: ELISA, IHC |
Recommended Dilutions: IHC: 25-100; ELISA: 1000-10000 |
Host Species: Rabbit |
Clonality: Rabbit Polyclonal |
Isotype: Immunogen-specific rabbit IgG |
Purification: Antigen affinity purification |
Species Reactivity: Human, Mouse, Rat |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
Research Areas: Cancer, Metabolism |
Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 219636(APAF1 Antibody) at a dilution of 1/25(Cytoplasm ). | In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the synthetic peptide and then with 219636(Anti-APAF1 Antibody) at dilution 1/25. | The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 219636(Anti-APAF1 Antibody) at a dilution of 1/25. | In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with synthetic peptide and then with D260053(Anti-APAF1 Antibody) at dilution 1/25. |
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