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AKT1 pAb

Anti-AKT1 Rabbit Polyclonal Antibody
5/5

¥2,600.00

Cat.#:  21274

   Size:   100 μl

In Stock

          Product Description          
AKT1 Polyclonal Antibody
货号:  21274
基因符号:  AKT1, AKT, PKB, PKB ALPHA, PRKBA, RAC, RAC ALPHA
描述:   AKT1 Rabbit Polyclonal Antibody
背景:   AKT1 is one of 3 closely related serine/threonine protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. Its function is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. There are evidences that mutation or abnormal expression in AKT1 is associated with cancers.
免疫原:   Purified AKT1 protein, human origin.
经过测试的应用:    ELISA, WB, IHC
推荐稀释度:    ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:200
浓度:   0.2mg/ml
种属反应性:   Rabbit
形式:   Liquid
克隆性:   Polyclonal
亚型:   IgG
纯化::   Purified from serum
Preservative: No
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Recognizes AKT1 of vertebrates.
储存条件::   Store at -20°C. Avoid repeated freezing and thawing
Western blot:   Western blot analysis of recombinant AKT1 protein. Purified His-tagged AKT1 protein was blotted with AKT1 rabbit polyclonal antibody (货号21274). Immunohistochemistry:   Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with AKT1 polyclonal antibody (货号21274). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat mouse IgG (1:50 dilution) was used as secondary antibody.
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