| 目录: 11098 |
| 产品名称: Human ADGRE5 Full Length Protein |
| 规格: 10 µg; 50 µg and 100 µg |
| 基因符号: CD97; TM7LN1 |
| Target: ADGRE5 |
| UNIPROT ID: P48960 |
| 描述: Human ADGRE5 Full Length Protein-Synthetic Nanodisc |
| 背景: A member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. |
| Species/Host: HEK293 |
| Molecular Weight: The human full length ADGRE5 protein has a MW of 90.5 kDa |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Protein Families: Druggable Genome, GPCR, Transmembrane |
| Protein Pathways: N/A |
Figure 1. Elisa plates were pre-coated with Flag Tag ADGRE5-Nanodisc (0.2μg/per well). Serial diluted Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for Flag monoclonal antibody binding with ADGRE5-Nanodisc is 7.327ng/ml. | 
Figure 2. Human ADGRE5-Nanodisc, Flag Tag on SDS-PAGE |
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