TP53(R175H)

货号:   26072

基因符号:  TP53(R175H)

描述:    TP53(R175H) 小鼠单克隆抗体

背景:   TP53 belongs to the p53 proteins family. It is ubiquitously expressed, acts as a tumor suppressor in many tumor types and induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Defects in TP53 are a cause of esophageal cancer (ESCR), lung cancer (LNCR), adrenocortical carcinoma (ADCC), choroid plexus papilloma (CPLPA) and so on.

免疫原:   A synthetic peptide from the internal region of TP53 which includes the mutation of R175H, human origin

经过测试的应用:   ELISA, WB, IF, IHC

推荐稀释度：    ELISA: 1:1000-1:5000 WB: 1:1000-1:5000 IHC: 1:50-1:100

浓度:    1 mg/ml

种属反应性:   Mouse

形式:   Liquid

克隆性：   Monoclonal

亚型:

纯化::   Purified from ascites

Preservative: No

成分:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

种属反应性:  TP53(R175H) antibody recognizes TP53(R175H) of vertebrates.

储存条件::  Store at -20°C. Avoid repeated freezing and thawing.

Western blot:   Western blot analysis of recombinant TP53(R175H) and wild type proteins. Purified His-tagged TP53(R175H) protein(目录10207) and wild type protein(目录10202) were blotted with TP53(R175H) monoclonal antibody(目录 26072). Immunofluorescence:   Immunofluorescence of cells expressing TP53 proteins with TP53(R175H) antibody. HEK293T cells were transfected with pCDNA3-GFP-TP53 (WT) plasmid (left column) or pCDNA3-GFP-TP53(R175H) plasmid (right column), then fixed and stained with TP53(R175H) monoclonal antibody (货号 26072).