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BRAF(G469E)

Anti BRAF(G469E) Mouse Monoclonal Antibody
5/5

¥4,800.00

Cat.#:  26196

   Size:   100 μl

In Stock

          Product Description          
BRAF(G469E)
货号:  26196
基因符号:  BRAF, BRAF1, RAFB1
描述:   BRAF(G469E) 小鼠单克隆抗体
背景:    BRAF belongs to a family of serine-threonine protein kinases. As a part of a signaling pathway known as the the RAS/MAPK pathway, it plays an important part in many cellular processes. The processes include cell proliferation, differentiation and transcriptional regulation. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers.
免疫原:   A synthetic peptide from the internal region of BRAF which includes the mutation of G469E, human origin.
经过测试的应用:   ELISA, WB, IHC
推荐稀释度:    ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100
浓度:   1mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯化::   Purified from ascites
Preservative: No
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Recognizes G469E mutant, but not wild type BRAF of vertebrates.
储存条件::   Store at -20°C. Avoid repeated freezing and thawing
Western blot:   Western blot analysis of recombinant BRAF (G469E) and wild type proteins. Purified His-tagged BRAF (G469E) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with BRAF(G469E) monoclonal antibody (cat. # 26196). Immunofluorescence:   Immunofluorescence of cells expressing BRAF proteins with BRAF(G469E) antibody. HEK293T cells were transfected with pCDNA3-GFP-BRAF (WT) plasmid (left column) or pCDNA3-GFP-BRAF (G469E) plasmid (right column), then fixed and stained with BRAF(G469E) monoclonal antibody (cat. # 26196).